Every living thing has a unique code written in DNA that has been evolving for billions of years. This DNA code is inherited by the offspring of an organism and it what mixes when we have babies. Since the discovery of DNA as the hereditary molecule and its beautiful structure(L) Scientists have been slowly gaining understanding and power over DNA and life itself.

In the 1980s Kary Mullis developed a way to amplify a few copies of any specific piece of DNA into millions of copies, in a method still used in every molecular biology laboratory around the world-PCR. This has transformed Molecular Biology, but PCR requires expensive equipment to rapidly and precisely change temperatures takes 2-3 hours and checking the results of a PCR is neither simple or intuitive or fast.

This is why we use Loop mediated Isothermal Amplification (LAMP). A related recent DNA amplification technique. LAMP is very specific and rapid, and allows amplification at a constant temperature, greatly simplifying the process and reducing cost. Specifically we use a new colorimetric  improvement which utilizes a pH sensitive dye and non buffered reaction  to allow seeing the results with the naked eye

 

Bibliography

  1. Watson, J. D., and F. H. Crick. 1953. “Genetical Implications of the Structure of Deoxyribonucleic Acid.” Nature 171 (4361): 964–67.
  2. Mullis, K., F. Faloona, S. Scharf, R. Saiki, G. Horn, and H. Erlich. 1986. “Specific Enzymatic Amplification of DNA in Vitro: The Polymerase Chain Reaction.” Cold Spring Harbor Symposia on Quantitative Biology 51 Pt 1: 263–73.
  3. Notomi, T., H. Okayama, H. Masubuchi, T. Yonekawa, K. Watanabe, N. Amino, and T. Hase. 2000. “Loop-Mediated Isothermal Amplification of DNA.” Nucleic Acids Research 28 (12): E63.
  4. Nagamine, K., T. Hase, and T. Notomi. 2002. “Accelerated Reaction by Loop-Mediated Isothermal Amplification Using Loop Primers.” Molecular and Cellular Probes 16 (3): 223–29.
  5. Tanner, Nathan A., Yinhua Zhang, and Thomas C. Evans Jr. 2015. “Visual Detection of Isothermal Nucleic Acid Amplification Using pH-Sensitive Dyes.” BioTechniques 58 (2): 59–68.
  6. Fukuta, Shiro, Yuko Mizukami, Akira Ishida, Junichi Ueda, Mayumi Hasegawa, Izumi Hayashi, Minako Hashimoto, and Michio Kanbe. 2004. “Real-Time Loop-Mediated Isothermal Amplification for the CaMV-35S Promoter as a Screening Method for Genetically Modified Organisms.” European Food Research and Technology = Zeitschrift Fur Lebensmittel-Untersuchung Und -Forschung. A 218 (5): 496–500.
  7. Feng, Jiawang, Shiming Tang, Lideng Liu, Xiaoshan Kuang, Xiaoyu Wang, Songnan Hu, and Shuzhu You. 2015. “Development of a Loop-Mediated Isothermal Amplification (LAMP) Assay for Rapid and Specific Detection of Common Genetically Modified Organisms (GMOs).” International Journal of Food Sciences and Nutrition 66 (2): 186–96.
  8. Lee, Meng-Shiou, Ting-Ying Su, Yi-Yang Lien, and Shyang-Chwen Sheu. 2017. “The Development of Loop-Mediated Isothermal Amplification (LAMP) Assays for the Rapid Authentication of Five Forbidden Vegetables in Strict Vegetarian Diets.” Scientific Reports 7 (March): 44238.